What question did this study set out to answer?

To evaluate the role of the V1B subunit of vacuolar ATPase, encoded by VMA2, in Candida albicans stress response, vacuolar biogenesis, autophagy, and virulence across different pH conditions.

July 19, 2014Eukaryotic CellOpen Access

The Contribution of Candida albicans Vacuolar ATPase Subunit V 1 B, Encoded by VMA2 , to Stress Response, Autophagy, and Virulence Is Independent of Environmental pH

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Authors

HRHallie S. RaneUniversity of New Mexico SBStella M. BernardoUniversity of New Mexico SHSummer R. HayekUniversity of New Mexico

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Implication

Experimental study demonstrates that VMA2-encoded vacuolar ATPase regulates stress response, autophagy, and virulence in Candida albicans, indicating pH-independent pathogenicity.

Key Points

To evaluate the role of the V1B subunit of vacuolar ATPase, encoded by VMA2, in Candida albicans stress response, vacuolar biogenesis, autophagy, and virulence across different pH conditions.
Constructed a tetracycline-repressible conditional mutant (tetR-VMA2) in Candida albicans to study the phenotypic effects of VMA2 inhibition.
Assessed growth under alkaline pH, osmotic stress, cold temperature, antifungal exposure, and nonfermentable carbon sources, while measuring proton transport, V-ATPase assembly, and autophagy.
Evaluated in vivo fungal virulence using a Caenorhabditis elegans nematode infection model.
VMA2 repression disrupted V-ATPase assembly, abolished proton transport, induced vacuolar alkalinization, and heightened sensitivity to osmotic stress, cold temperatures, calcium, and antifungal drugs.
Repression of VMA2 markedly impaired key virulence traits, including hyphal filamentation, degradative enzyme secretion, and autophagy under nitrogen starvation.
The tetR-VMA2 mutant was completely avirulent in the acidic in vivo C. elegans model, indicating that V-ATPase function is required for pathogenesis independently of environmental pH.

Cite This Study

Rane et al. (2014) studied this question.

synapsesocial.com/papers/6a8137bdba5888e95f4d7e03 https://doi.org/10.1128/ec.00135-14

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Implication

Experimental study demonstrates that VMA2-encoded vacuolar ATPase regulates stress response, autophagy, and virulence in Candida albicans, indicating pH-independent pathogenicity.

The Contribution of Candida albicans Vacuolar ATPase Subunit V 1 B, Encoded by VMA2 , to Stress Response, Autophagy, and Virulence Is Independent of Environmental pH

Discussion

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Implication

Key Points

Cite This Study

The Contribution of Candida albicans Vacuolar ATPase Subunit V 1 B, Encoded by VMA2 , to Stress Response, Autophagy, and Virulence Is Independent of Environmental pH

Discussion

Member takes

Implication

Key Points

Cite This Study

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