Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 1, 1990Molecular and Cellular Biology

Role of Vacuolar Acidification in Protein Sorting and Zymogen Activation: a Genetic Analysis of the Yeast Vacuolar Proton-Translocating ATPase

View Full Paper
Ask AI
Bookmark
Share

Why the study?

Does mutation of the VAT2 gene affect vacuolar acidification, protein sorting, and zymogen activation in yeast?

Population

Yeast cells

Comparison

Mutation of the VAT2 gene (vat2 null allele) vs Wild-type yeast cells

Design

Preclinical

Authors

CYCarl T. YamashiroArizona State UniversityPKPatricia M. KaneSUNY Upstate Medical UniversityDWDavid F WolczykUniversity of Oregon

Discussion

Loading...

Member takes

Implication

VAT2 deletion abolishes vacuolar acidification and growth in yeast; leaves open relevance to mammalian V-ATPase biology.

Structured PICO

Does mutation of the VAT2 gene affect vacuolar acidification, protein sorting, and zymogen activation in yeast?

P
Population
Yeast cells
I
Intervention
Mutation of the VAT2 gene (vat2 null allele)
C
Comparator
Wild-type yeast cells
O
Outcome
Vacuolar acidification (pH), growth in neutral pH medium, vacuolar ATPase activity, zymogen activation, and vacuolar protein sortingsurrogate

The vacuolar proton-translocating ATPase complex is essential for vacuolar acidification and normal growth in neutral pH, but not strictly required for zymogen activation.

Cite This Study

Yamashiro et al. (1990) studied this question.

synapsesocial.com/papers/6a76bc20f6b553d308ae020bhttps://doi.org/10.1128/mcb.10.7.3737-3749.1990
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A thermosensitive lesion in a Chinese hamster cell mutant causing differential effects on the acidification of endosomes and lysosomes.1986 · 29 citations
  2. 2Lyticase: Endoglucanase and Protease Activities That Act Together in Yeast Cell Lysis1980 · 312 citations
  3. 3Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting.1988 · 414 citations
  4. 4Protein sorting in yeast: the role of the vacuolar protontranslocating ATPase1989 · 18 citations
  5. 5A Conserved Gene Encoding the 57-kDa Subunit of the Yeast Vacuolar H+-ATPase1989 · 144 citations