Key result
Acute pharmacological or physiological inhibition of V-ATPase pump activity de-acidifies the vacuole lumen and induces vacuole fusion in living yeast cells, indicating that proton pump activity negatively regulates fusion.
Population
Yeast cells (Saccharomyces cerevisiae, including wildtype BY4741 and various V-ATPase mutants)
Comparison
Acute pharmacological or physiological… vs Wildtype yeast cells or untreated cells
Design
Preclinical
Authors
Loading...
Hypothesis-generating in yeast; leaves open V-ATPase role in mammalian cardiomyocyte vacuolar fusion.
In yeast, proton pump activity of the V-ATPase negatively regulates vacuole fusion in vivo, while the physical presence of the V0 sector is required for fusion.
Desfougères et al. (2016) studied Yeast vacuole membrane fusion. V-ATPase inhibition (Concanamycin A or glucose withdrawal) vs. Untreated cells was evaluated on Vacuole fusion (assessed by time-lapse microscopy). Acute pharmacological or physiological inhibition of V-ATPase pump activity de-acidifies the vacuole lumen and induces vacuole fusion in living yeast cells, indicating that proton pump activity negatively regulates fusion.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: