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Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si(111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity.
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Ming-liang et al. (2008) studied this question.
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