What question did this study set out to answer?

To systematically map and deconstruct the proteome-wide substrate specificities of individual O-linked mannose glycosyltransferase enzyme families in human cells.

January 16, 2024Open Access

Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells

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Authors

LPLorenzo PovoloUniversity of Copenhagen WTWeihua TianTechnical University of Denmark SVSergey Y. VakhrushevUniversity of Copenhagen

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Implication

Glycoproteomic profiling reveals distinct substrate specificities across O-mannose transferase pathways in human cell lines, highlighting targeted modification of cell-surface Ig-like domains.

Key Points

To systematically map and deconstruct the proteome-wide substrate specificities of individual O-linked mannose glycosyltransferase enzyme families in human cells.
Generated a human cell library across five cell lines carrying individual and combinatorial knockouts of POMT1/POMT2, TMTC1-4, and TMEM260 glycosyltransferase genes.
Applied targeted quantitative membrane glycoproteomics workflows to profile pathway-specific initiation and substrate targets.
Identified 180 O-mannose glycoproteins and uncovered novel target substrates modified specifically by the POMT1/POMT2 initiation pathway.
Demonstrated that TMTC1-4 and TMEM260 pathways broadly target distinct immunoglobulin-like (Ig-like) domains on plasma membrane proteins involved in cell-cell and cell-extracellular matrix adhesion.

Cite This Study

Povolo et al. (2024) studied this question.

synapsesocial.com/papers/6a815e7fa0713f3198f09825 https://doi.org/10.1101/2024.01.15.575371

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Implication

Glycoproteomic profiling reveals distinct substrate specificities across O-mannose transferase pathways in human cell lines, highlighting targeted modification of cell-surface Ig-like domains.

Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Global view of domain-specific O-linked mannose glycosylation in glycoengineered cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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