What question did this study set out to answer?

To investigate how the neutral metalloprotease inhibitor phosphoramidon alters vascular contractile responses to angiotensin II, endothelin peptides, and vasopressin across different rat blood vessels.

April 1, 1995British Journal of PharmacologyOpen Access

Differential effects of phosphoramidon on contractile responses to angiotensin II in rat blood vessels

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Population

Endothelium intact and denuded ring preparations of aorta, mesenteric artery, and tail artery isolated from…

Comparison

Phosphoramidon at various concentrations vs Absence of phosphoramidon

Design

Preclinical

Authors

LCLihua ChenJiangxi University of Traditional Chinese Medicine JMJ. Robert McNeillUniversity of British Columbia Hospital TWThomas W. WilsonUniversity of Canterbury

Discussion

Member takes

Implication

These rat artery findings are hypothesis-generating for endothelin-angiotensin interactions; should not yet change clinical practice or prompt human trials.

Key Points

To investigate how the neutral metalloprotease inhibitor phosphoramidon alters vascular contractile responses to angiotensin II, endothelin peptides, and vasopressin across different rat blood vessels.
Measured concentration-tension curves for angiotensin II, big ET-1, ET-1, and AVP in isolated aorta, mesenteric artery, and tail artery rings from adult male Sprague-Dawley rats.
Evaluated the inhibitory effects of phosphoramidon (10–100 µM) across vascular preparations with intact versus denuded endothelium.
Phosphoramidon (10 µM) significantly increased EC50 values for angiotensin II in the tail artery (P < 0.01) and mesenteric artery (P < 0.05), but did not alter responses in the aorta or responses to ET-1 and AVP.
Endothelial removal completely abolished angiotensin II-induced contractions in the tail artery, reduced responsiveness in the mesenteric artery, and shifted sensitivity leftward in the aorta.

Structured PICO

Population

Endothelium intact and denuded ring preparations of aorta, mesenteric artery, and tail artery isolated from adult male Sprague-Dawley rats

Intervention

Phosphoramidon (neutral metalloprotease inhibitor) at various concentrations (e.g., 10 microM, 100 microM)

Comparator

Absence of phosphoramidon

Outcome

Cumulative concentration-tension response (C-R) curves to angiotensin II (AII), big endothelin-1 (big ET-1), ET-1, and arginine vasopressin (AVP)surrogate

Vasoconstrictor responses to angiotensin II in mesenteric and tail arteries appear to be mediated by the release of endothelins from the endothelium via increased formation from big ET, an effect blocked by phosphoramidon.

Cite This Study

Chen et al. (1995) studied this question.

synapsesocial.com/papers/6a815e8fadfa6baaaee1cf5d https://doi.org/10.1111/j.1476-5381.1995.tb14945.x

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Implication

These rat artery findings are hypothesis-generating for endothelin-angiotensin interactions; should not yet change clinical practice or prompt human trials.

Differential effects of phosphoramidon on contractile responses to angiotensin II in rat blood vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Differential effects of phosphoramidon on contractile responses to angiotensin II in rat blood vessels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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