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August 16, 2026Pest Management Science

Allelic dosage across four functional ALS loci shapes ALS ‐inhibitor resistance in allotetraploid Pontederia vaginalis

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Authors

KOKensuke OhtaYFYoshimi FujinoYSYoshinao Sada

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Overview

Genetic cross and dose-response analysis demonstrates dosage-dependent herbicide resistance in Pontederia vaginalis, suggesting uncertain weed control outcomes at standard field rates.

Key Points

  • To determine how resistant allelic dosage across multiple functional ALS loci impacts whole-plant herbicide susceptibility in allotetraploid Pontederia vaginalis.
  • Crossed P. vaginalis lineages carrying Pro197Ser substitutions in either MvALS1 or MvALS3 to generate F2 progeny with variable allelic dosages.
  • Genotyped progeny to verify independent locus segregation and conducted dose-response assays using imazosulfuron and bensulfuron-methyl.
  • Susceptibility decreased sharply from zero to two resistant alleles, shifting the imazosulfuron ED90 ~3000-fold, whereas exceeding two alleles provided only a marginal ~4- to 5-fold increase in resistance.
  • For plants confirmed with one resistant allele (n = 25), the bootstrap 95% CI for ED90 was 16.7–292.0 g a.i. ha−1 (median: 92.6 g a.i. ha−1), overlapping the standard field dose (90 g a.i. ha−1) and distinct from zero- and two-allele classes.
  • F2 segregation ratios aligned with Mendelian expectations, indicating that non-target-site resistance mechanisms did not significantly alter inheritance patterns.

Cite This Study

Ohta et al. (2026) studied this question.

synapsesocial.com/papers/6a8179bcf2fb91fc834ad159https://doi.org/10.1002/ps.71188
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