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June 1, 2008European Journal of Cardiovascular Prevention & RehabilitationOpen Access

Modulation of Murf-1 and MAFbx expression in the myocardium by physical exercise training

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Key result

Four weeks of exercise training significantly reduced the myocardial expression of Murf-1 (1.2 vs 5.9 arb. units), MAFbx (6.4 vs 84.0 arb. units), and TNF-alpha in rats post-myocardial infarction.

Why the study?

Does physical exercise training reduce myocardial expression of Murf-1 and MAFbx in rats after myocardial infarction?

Population

Rats, 3 weeks after left anterior descending artery ligation (myocardial infarction) or sham operation

Comparison

4-week physical exercise training vs Inactive control group

Design

Preclinical

Follow-up

4 weeks

Authors

VAVolker AdamsALAxel LinkeSGStephan Gielen

Discussion

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Overview

May attenuate post-MI atrophy pathways in rats; hypothesis-generating and should not change practice.

Key Points

  • To investigate the effect of physical exercise training on the myocardial expression of atrophy-related E3 ubiquitin ligases Murf-1 and MAFbx after myocardial infarction.
  • Rats underwent left anterior descending artery ligation to induce myocardial infarction or a sham operation, and three weeks post-surgery were assigned to either a four-week exercise training program or an inactive control group.
  • Remote myocardium tissue was collected to assess Murf-1 and MAFbx expression via quantitative real-time PCR and western blot, and myocardial TNF-alpha concentrations via ELISA.
  • Seven weeks post-infarction, inactive rats demonstrated significant upregulation in myocardial mRNA expression of Murf-1 (sham: 1.8±0.3 vs. MI: 5.9±1.2 arb. units; P<0.05), MAFbx (sham: 13.9±2.4 vs. MI: 84.0±22.7 arb. units; P<0.05), and local TNF-alpha concentration (sham: 322±48 vs. MI: 1010±170 pg/mg; P<0.001).
  • Four weeks of exercise training significantly reduced levels compared to inactive controls for Murf-1 (1.2±0.6 arb. units), MAFbx (6.4±2.6 arb. units), and TNF-alpha (419±64 pg/mg), with Murf-1 and MAFbx expression negatively correlating with myocardial function.

Structured PICO

Does physical exercise training reduce myocardial expression of Murf-1 and MAFbx in rats after myocardial infarction?

P
Population
Rats 3 weeks post-myocardial infarction or sham operation, subjected to 4 weeks of exercise training or inactivity.
I
Intervention
4-week physical exercise training
C
Comparator
Inactive control group
O
Outcome
Myocardial expression of Murf-1 and MAFbx (evaluated by quantitative real time PCR and western blot) and myocardial concentration of TNF-alphasurrogate

Main Result

Absolute Event Rate: 1.2% vs 5.9%

Exercise training attenuates the upregulation of atrophy-related E3 ubiquitin ligases Murf-1 and MAFbx in the myocardium after myocardial infarction in a rat model.

Cite This Study

Adams et al. (2008) studied Myocardial infarction / Heart failure. Exercise training vs. Inactive control was evaluated on Myocardial expression of Murf-1. Four weeks of exercise training significantly reduced the myocardial expression of Murf-1 (1.2 vs 5.9 arb. units), MAFbx (6.4 vs 84.0 arb. units), and TNF-alpha in rats post-myocardial infarction.

synapsesocial.com/papers/6a819140d433ecbb06e1d4c9https://doi.org/10.1097/hjr.0b013e3282f3ec43
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