October 1, 1999Journal of Biological ChemistryOpen Access

Role of the N-terminal Region of the Regulatory Light Chain in the Dephosphorylation of Myosin by Myosin Light Chain Phosphatase

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Population

In vitro biochemical model studying myosin regulatory light chain and heterotrimeric myosin light chain…

Comparison

Deletion of N-terminal amino acid residues of RLC vs Intact RLC or isolated RLC

Design

Preclinical

Authors

RIReiko IkebeThe University of Texas Health Science Center at Tyler SRS ReardonUniversity of Massachusetts Chan Medical School TMToshiaki MitsuiNiigata University

Discussion

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Implication

Animal data on RLC N-terminus leave open its role in human myosin phosphatase activity; no practice change warranted.

Structured PICO

Population

In vitro biochemical model studying myosin regulatory light chain (RLC) and heterotrimeric myosin light chain phosphatase (MLCP)

Intervention

Deletion of N-terminal amino acid residues (seven or four) of RLC

Comparator

Intact RLC or isolated RLC

Outcome

Dephosphorylation rate of specific RLC phosphorylation sites (phosphoserine 19, phosphothreonine 18, phosphothreonine 9, and serine 1/serine 2)surrogate

The N-terminal region of the myosin regulatory light chain is crucial for substrate recognition by myosin light chain phosphatase during myosin dephosphorylation.

Cite This Study

Ikebe et al. (1999) studied this question.

synapsesocial.com/papers/6a81ead8bcfc941b47bf756c https://doi.org/10.1074/jbc.274.42.30122

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Role of the N-terminal Region of the Regulatory Light Chain in the Dephosphorylation of Myosin by Myosin Light Chain Phosphatase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Role of the N-terminal Region of the Regulatory Light Chain in the Dephosphorylation of Myosin by Myosin Light Chain Phosphatase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider