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July 1, 1990The Journal of Cell BiologyOpen Access

Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells.

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Population

Mammalian fibroblasts (living nonmuscle cells)

Comparison

Microinjection of purified type-1 phosphatase… vs Microinjection of equivalent amounts of purified…

Design

Preclinical

Follow-up

within 30 min

Authors

AFAnne FernandezCentre National de la Recherche ScientifiqueDBDavid L. BrautiganUniversity of VirginiaMMMarc C. MumbyUniversity of California, Riverside

Discussion

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Implication

Highlights type-1 phosphatase in cytoskeletal control; leaves open its role in human cardiovascular pathophysiology.

Structured PICO

P
Population
Mammalian fibroblasts (living nonmuscle cells)
I
Intervention
Microinjection of purified type-1 phosphatase into the cytoplasm
C
Comparator
Microinjection of equivalent amounts of purified type-2A phosphatase
O
Outcome
Actin microfilament network organization and myosin light chain phosphorylationsurrogate

Type-1 phosphatase, but not type-2A, plays a predominant role in the dephosphorylation of myosin light chain and modulation of actin microfilament organization in living nonmuscle cells.

Cite This Study

Fernandez et al. (1990) studied this question.

synapsesocial.com/papers/6a81ead8bcfc941b47bf7576https://doi.org/10.1083/jcb.111.1.103
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES1989 · 2,547 citations
  2. 2Regulation of Actin-Myosin Interaction by Reversible Phosphorylation of Myosin and Myosin Kinase1982 · 28 citations
  3. 3Purification and characterization of catalytic subunit of skeletal muscle adenosine 3':5'-monophosphate-dependent protein kinase.1977 · 326 citations
  4. 4Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase.1988 · 278 citations
  5. 5Purification and characterization of a multisubunit phosphatase from turkey gizzard smooth muscle. The effect of calmodulin binding to myosin light chain kinase on dephosphorylation.1983 · 120 citations