Indomethacin Inhibition of Bumetanide Diuresis in Dogs

Clearance experiments in conscious, trained, intact dogs and also a one minute urine collection in conscious, ureterostomized dogs show that indomethacin pretreatment significantly alters the renal haemodynamic end excretional effects of bumetanide. The haemodynamic parameters (inulin and PAH clearance) are significantly reduced during the time interval 10–40 minutes after bumetanide administration in indomethacin pretreated dogs. In the same conditions urine volume, and sodium and chloride excretion are reduced while potassium excretion is less influenced. Indomethacin pretreatment does not affect the abolition of the cortico‐medullary electrolyte gradient caused by bumetanide administration. The results support evidence for an important role of prostaglandins in the diuretic effect of bumetanide.