October 1, 1991Cardiovascular Research
Release of endothelium derived nitric oxide in relation to pressure and flow
Population
Porcine macrovascular endothelial cells cultured on microcarrier beads
Comparison
Perfusion at different flow rates and perfusion… vs Different levels of flow and pressure
Design
Preclinical
Authors
Discussion
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Implication
Flow modulates endothelial NO release independently of pressure in this model; leaves open relevance to intact human vessels.
Key Points
- To investigate how the rate of endothelium-derived nitric oxide (NO) release is modulated by two primary determinants of vascular conductance: perfusion pressure and flow rate.
- Perfused packed columns of porcine macrovascular endothelial cells cultured on microcarrier beads at varied flow rates (2, 10, and 20 ml·min⁻¹) and perfusion pressures (6 to 200 mm Hg).
- Continuously quantified NO release rates under basal and ATP-stimulated (10⁻⁴ M) conditions using a difference spectrophotometric assay.
- Increasing flow rate from 2 to 20 ml·min⁻¹ increased basal NO release 5-fold and increased ATP-stimulated NO release 5.8-fold (>180 pmol·min⁻¹·mg⁻¹ endothelial cell protein).
- ATP stimulation augmented endothelial NO release more than 10-fold at every flow level tested.
- Varying perfusion pressure between 6 and 200 mm Hg produced no change in the rate of basal NO release.
Structured PICO
P
Population
Porcine macrovascular endothelial cells cultured on microcarrier beads
I
Intervention
Perfusion at different flow rates (2, 10, 20 ml.min-1) and perfusion pressures (ranging from 6 to 200 mm Hg), under basal and ATP (10(-4) M) stimulated conditions
C
Comparator
Different levels of flow and pressure
O
Outcome
Rate of endothelium derived nitric oxide (NO) releasesurrogate
Endothelial nitric oxide release is highly responsive to changes in flow but not to changes in perfusion pressure.
Cite This Study
Kelm et al. (1991) studied this question.