August 3, 2017Civil War Book ReviewOpen Access

Systemic Sympathoexcitation Was Associated with Paraventricular Hypothalamic Phosphorylation of Synaptic CaMKIIα and MAPK/ErK

View Full Paper

Ask AI

Bookmark

Why the study?

Does PVN-specific TLR4 knockdown attenuate the alteration of synaptic kinases (CaMKIIα and MAPK/ErK) induced by systemic isoproterenol in mice?

Population

Adult C57BL/6, transgenic C57BL/TLR4loxp/loxp, and C57BL-Venus mice, weighing 22-25 g.

Comparison

Isoproterenol 150 mg/kg intraperitoneally for… vs Normal saline or control vector.

Design

Preclinical

Follow-up

48 hours

Authors

OOOlalekan M. OgundeleLouisiana State University FRFernando Azadinho RosaUniversidade Estadual Paulista (Unesp)RDRohan DharmakumarCardiac Imaging

Discussion

Member takes

Implication

Clinical meaning: Cannot change practice, purely mechanistic/hypothesis-generating regarding sympathoexcitation.

Structured PICO

Does PVN-specific TLR4 knockdown attenuate the alteration of synaptic kinases (CaMKIIα and MAPK/ErK) induced by systemic isoproterenol in mice?

Population

Adult C57BL/6 (WT; n=20), transgenic C57BL/TLR4loxp/loxp (n=20), and C57BL (VGAT)-Venus mice (n=10), weighing 22-25 g.

Intervention

Isoproterenol (ISOP) 150 mg/kg intraperitoneally (two injections at 24h interval) for 48h, with or without PVN-specific knockdown of TLR4 using AAV-CMV-Cre-eGFP.

Comparator

Normal saline (i.p.) or control vector (AAV-CMV-eGFP).

Outcome

Expression and phosphorylation of PVN CaMKIIα and MAPK/ErK, and TLR4 expression.surrogate

Systemic adrenergic activation alters pre-autonomic synaptic kinases downstream of TLR4, and PVN-specific TLR4 knockdown attenuates these sympathoexcitatory effects.

Cite This Study

Ogundele et al. (2017) studied this question.

synapsesocial.com/papers/6a821da48ef2ccdd49f25242 https://doi.org/10.3389/fnins.2017.00447

View Full Paper

Ask AI

Bookmark

Systemic Sympathoexcitation Was Associated with Paraventricular Hypothalamic Phosphorylation of Synaptic CaMKIIα and MAPK/ErK

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Systemic Sympathoexcitation Was Associated with Paraventricular Hypothalamic Phosphorylation of Synaptic CaMKIIα and MAPK/ErK

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider