A Study of the Multiplicity of Lysine-rich Histones

This is the authors' abstract. We don't add key points for this paper.

Fractions of lysine-rich histones have been obtained from rabbit thymus and mammary gland by chromatography on Amberlite IRC-50 with shallow guanidinium chloride gradients. The peaks obtained were rechromatographed using still shallower gradients. Only one of nine peaks was found to be significantly inhomogeneous on rechromatography. Furthermore, each of the other eight peaks was as narrow as a chromatographically homogeneous sample and each was electrophoretically homogeneous on polyacrylamide gel. All fractions from the thymus possessed blocked aminoterminal residues and were terminated at the carboxyl end by 2 adjacent lysine residues. It was concluded that this system of chromatography had been extended nearly to its limit of resolving power and that the complexity of these chromatographic patterns approximates the true multiplicity of lysine-rich histones.