What question did this study set out to answer?

To identify the specific structural domains of the lasso peptide microcin J25 responsible for interacting with and inhibiting bacterial RNA polymerase.

May 18, 2005Journal of BacteriologyOpen Access

Structure-Activity Analysis of Microcin J25: Distinct Parts of the Threaded Lasso Molecule Are Responsible for Interaction with Bacterial RNA Polymerase

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Authors

ESEkaterina SemenovaRutgers, The State University of New Jersey YYYulia YuzenkovaNewcastle College JPJean PéduzziWayne State University

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Implication

In vitro structural analysis reveals truncated microcin J25 inhibits bacterial RNA polymerase, indicating separate regions mediate transcription inhibition and cellular uptake.

Key Points

To identify the specific structural domains of the lasso peptide microcin J25 responsible for interacting with and inhibiting bacterial RNA polymerase.
Assayed transcription inhibition using intact microcin J25 (MccJ25), thermolysin-cleaved MccJ25, and an engineered MccJ25 mutant lacking amino acids 13 to 17.
Performed comparative structural analyses on intact and thermolysin-digested forms of MccJ25.
Thermolysin-cleaved MccJ25 and mutant MccJ25 lacking residues 13 to 17 successfully inhibited bacterial RNA polymerase transcription.
Structural comparison revealed that transcription inhibition and bacterial cell entry are mediated by distinct regions of the threaded lasso molecule.

Cite This Study

Semenova et al. (2005) studied this question.

synapsesocial.com/papers/6a82472b5a62cdcac13f16b2 https://doi.org/10.1128/jb.187.11.3859-3863.2005

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Implication

In vitro structural analysis reveals truncated microcin J25 inhibits bacterial RNA polymerase, indicating separate regions mediate transcription inhibition and cellular uptake.

Structure-Activity Analysis of Microcin J25: Distinct Parts of the Threaded Lasso Molecule Are Responsible for Interaction with Bacterial RNA Polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

Structure-Activity Analysis of Microcin J25: Distinct Parts of the Threaded Lasso Molecule Are Responsible for Interaction with Bacterial RNA Polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

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