Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction

Figures

• 

  Figure 1.. Myocardial infarct size and hematoxylin and eosin (HE) staining. (A) Normal myocardium is stained red, while pale grey areas indicate infarct areas. (B) HE staining was conducted to detect the pathological alterations (magnification, ×400). S, sham group; M, model group; GL, low dosage group; GM, medium dosage group; GH, high dosage group.

• 

  Figure 2.. Effect of Guanmaitong (GMT) on cardiac marker enzyme activity in rats. Treatment with GMT attenuated the elevation of cardiac marker enzyme activity, and different dose groups of GMT decline to different extent. #P<0.05 vs. sham group; *P<0.05 vs. model group, as determined by one-way analysis of variance followed by Dunnett's test. M, model group; S, sham group; GL, low dosage group; GM, medium dosage group; GH, high dosage group; CK, creatine kinase; LDH, lactate dehydrogenase.

• 

  Figure 3.. Expression of Bcl-2 and Bax in GMT-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western Blot was used to evaluate the protein expression of Bcl-2 and Bax. (C) Immunohistochemical analysis was used to detected the expression of Bcl-2 and Bax (magnification, ×400). #P<0.05 vs. sham group; *P<0.05 vs. model group, as determined by one-way analysis of variance followed by Dunnett's test. S, sham group; M, model group; GL, low dosage group; GM, medium dosage group; GH, high dosage group; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein.

• 

  Figure 4.. Detection of inflammation-related cytokine expression using reverse transcription-quantitative polymerase chain reaction. #P<0.05 vs. sham group; *P<0.05 vs. model group, as determined by one-way analysis of variance followed by Dunnett's test. M, model group; S, sham group; GL, low dosage group; GM, medium dosage group; GH, high dosage group; IL-1, interleukin-1; TNF-α, tumor necrosis factor-α; ICAM-1, intercellular adhesion molecule 1.

• 

  Figure 5.. Detection of inflammation-related cytokine proteins in Guanmaitong-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western blot was used to analyze the protein expression levels of ICAM-1 and TNF-α. (C) IL-1 expression was analyzed using an ELISA assay. #P<0.05 vs. sham group; *P<0.05 vs. model group, as determined by one-way analysis of variance followed by Dunnett's test. M, model group; S, sham group; GL, low dosage group; GM, medium dosage group; GH, high dosage group; ICAM-1, intercellular adhesion molecule 1; TNF-α, tumor necrosis factor-α; IL-1, interleukin-1.