Identification of key genes associated with cellular aging and mitochondria in acute myocardial infarction

Figures

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  Fig. 1. Identification of DEGs and key modules gene between AMI and control group. (a) Volcano map of differentially expressed genes. (b) Differentially expressed gene heat map. (c) Differences in ssGSEA scores for CARGs. (d) After introducing sample traits, sample hierarchical clustering. (e) Soft threshold screening. (f) Identification of co-expression module. (g) ssGSEA Score Correlation Heat Map for Key Modules and CARGs.

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  Fig. 2. Discovery and functional enrichment exploration of 37 candidate genes. (a) Candidate gene identification. (b) GO enrichment results of candidate genes. (c) KEGG Enrichment Results and Chord Plot for Candidate Genes. (d) PPI map of candidate genes.

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  Fig. 3. IDI1 and PMAIP1 as key genes for AMI through LASSO, SVM-RFE, and ROC analysis. (a) Lasso analysis. (b) SVM-RFE accuracy. (c) Wayne Plot Results for Characteristic Genes. (d) Expression verification. (e) ROC curves of characteristic genes in Training-GSE66360 (left) and Test-GSE29532 (right).

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  Fig. 4. Construction and evaluation of nomograms. (a) Biomarker construction nomogram model. (b) Nomogram model calibration curve. (c) ROC Curve of Nomogram Model. (d) DCA curve for nomogram model.

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  Fig. 5. Marked variations in immune cell infiltration and subcellular localization of IDI1 and PMAIP1 in AMI. (a) Immune infiltration cell enrichment fraction based on heat map between AMI group and control group. (b) Box plot of the difference in enrichment fraction of 28 immunoinfiltrating cells between AMI group and Control group. (c) Heat map of correlation between biomarkers and differential immune cells. (d) Biomarker subcellular localization.

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  Fig. 6. Molecular regulatory network revealed key interactions for IDI1 and PMAIP1. (a) TF-miRNA-mRNA regulatory network. (b) mRNA-miRNA-lncRNA regulatory network.

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  Fig. 7. Drug prediction and molecular docking reveal strong binding affinity of cyclosporine with IDI1 and PMAIP1. (a) Biomarker drug network map. (b) Molecular Docking of IDI1 and Cyclosporine.

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  Fig. 8. Expression levels of IDI1 and PMAIP1 in control and AMI groups by RT-qPCR. (a) Relative IDI1 lever to GAPDH. (b) Relative PMAIP1 lever to GAPDH.