What question did this study set out to answer?

To measure the activities of CPT-11 converting carboxylesterase and topoisomerase I in colorectal carcinomas, liver metastases, and adjacent normal tissues.

April 9, 1999British Journal of CancerOpen Access

CPT-11 converting carboxylesterase and topoisomerase I activities in tumour and normal colon and liver tissues

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Authors

SGSylvie M. GuichardNovo Nordisk (United Kingdom)CTC. TerretCentre Léon Bérard IHIsabelle HennebelleInstitut Claudius Regaud

Discussion

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Implication

Tissue analysis reveals wide variations in carboxylesterase and topoisomerase I levels in colorectal cancer, suggesting potential local activation of CPT-11 chemotherapy.

Key Points

To measure the activities of CPT-11 converting carboxylesterase and topoisomerase I in colorectal carcinomas, liver metastases, and adjacent normal tissues.
Assayed tissue samples from 53 colorectal tumors, 8 liver metastases, and matched adjacent normal tissues.
Quantified enzyme activities for CPT-11 converting carboxylesterase (CPT-CE) and topoisomerase I (topo I).
CPT-CE activity was two- to threefold lower in primary tumors compared to normal liver, while displaying similar activity in normal colon and liver tissues.
Tumor topo I activity ranged from 580 to 84,900 U/mg protein, exceeding 40,000 U/mg protein in 11 of 53 patients, with a tumor-to-normal ratio greater than 5 in 12 of 53 patients.
An inverse correlation occurred between topoisomerase I activity and the clinical stage of colorectal disease.

Cite This Study

Guichard et al. (1999) studied this question.

synapsesocial.com/papers/6a828704aa66c98ddba0bcd8 https://doi.org/10.1038/sj.bjc.6690364

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Implication

Tissue analysis reveals wide variations in carboxylesterase and topoisomerase I levels in colorectal cancer, suggesting potential local activation of CPT-11 chemotherapy.

CPT-11 converting carboxylesterase and topoisomerase I activities in tumour and normal colon and liver tissues

Discussion

Member takes

Implication

Key Points

Cite This Study

CPT-11 converting carboxylesterase and topoisomerase I activities in tumour and normal colon and liver tissues

Discussion

Member takes

Implication

Key Points

Cite This Study

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