What question did this study set out to answer?

To determine whether endothelium-dependent vasorelaxation induced by various agonists is linked to calcium-activated potassium efflux in aortic endothelial cells.

June 1, 1983British Journal of PharmacologyOpen Access

Endothelium‐dependent relaxation of the pig aorta: relationship to stimulation of 86Rb efflux from isolated endothelial cells

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Population

Pig aortic strips (histamine-contracted) and isolated pig aortic endothelial cells

Design

Preclinical

Authors

JGJohn L. GordonQatar Petroleum (Qatar)WMWilliam MartinMarquette University

Discussion

Member takes

Implication

Acetylcholine elicits endothelium-dependent relaxation without 86Rb efflux in pig aorta; leaves open distinct K+ pathways and requires human translation studies.

Key Points

To determine whether endothelium-dependent vasorelaxation induced by various agonists is linked to calcium-activated potassium efflux in aortic endothelial cells.
Assessed dose-dependent relaxation in histamine-contracted pig aortic strips with and without endothelium exposed to bradykinin, ATP, acetylcholine, and the calcium ionophore A23187.
Measured 86Rb efflux from isolated preloaded pig aortic endothelial cells in response to vasodilators and the calcium influx blocker lanthanum.
Bradykinin, ATP, ADP, and A23187 stimulated dose-dependent 86Rb efflux with potencies closely matching their endothelium-dependent relaxant effects, which were abolished by lanthanum.
Acetylcholine and carbachol induced robust endothelium-dependent aortic relaxation without stimulating 86Rb efflux from isolated endothelial cells.

Structured PICO

Population

Pig aortic strips (histamine-contracted) and isolated pig aortic endothelial cells

Intervention

Bradykinin, ATP, acetylcholine, ADP, AMP, adenosine, carbachol, A23187, and lanthanum

Outcome

Endothelium-dependent relaxation and 86Rb effluxsurrogate

Endothelium-dependent relaxation induced by bradykinin and ATP, but not acetylcholine, is associated with calcium-activated potassium efflux in pig aortic endothelial cells.

Cite This Study

Gordon et al. (1983) studied this question.

synapsesocial.com/papers/6a828cc29f5484a76e0f6232 https://doi.org/10.1111/j.1476-5381.1983.tb11028.x

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Implication

Acetylcholine elicits endothelium-dependent relaxation without 86Rb efflux in pig aorta; leaves open distinct K+ pathways and requires human translation studies.

Endothelium‐dependent relaxation of the pig aorta: relationship to stimulation of 86Rb efflux from isolated endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Endothelium‐dependent relaxation of the pig aorta: relationship to stimulation of 86Rb efflux from isolated endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider