Changes in Dihydrofolate Reductase (DHFR) mRNA Levels Can Account Fully for Changes in DHFR Synthesis Rates during Terminal Differentiation in a Highly Amplified Myogenic Cell Line

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Population

Mouse muscle cell line resistant to 300 microM methotrexate (H- alpha R300T cells)

Comparison

Myogenic withdrawal from the cell cycle vs Proliferative cells

Design

Preclinical

Follow-up

15 to 24 h

Authors

Discussion

Transcriptional control of DHFR dominates in animal differentiation models; leaves open relevance to human cardiovascular cell maturation.

Population

Mouse muscle cell line resistant to 300 microM methotrexate (H- alpha R300T cells)

Intervention

Myogenic withdrawal from the cell cycle (terminal differentiation)

Comparator

Proliferative cells

Outcome

DHFR mRNA levels and DHFR synthesis ratessurrogate

Replication-dependent changes in DHFR synthesis rates during terminal differentiation are determined exclusively by changes in DHFR mRNA levels.

Schmidt et al. (1991) studied this question.

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Transcriptional control of DHFR dominates in animal differentiation models; leaves open relevance to human cardiovascular cell maturation.