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A novel method is described for the production of saline-reacting antibodies from "incomplete' blood grouping sera. IgG anti-D is combined to protein A-bearing Staphylococcus aureus (SA), forming a polyvalent anti-D assembly. The SA-anti-D has a high titre in saline and is stable on 4 degrees C storage and lyophilisation. The material was successful in Rh(D) typing cells sensitised in vitro with various antibodies. Saline-reacting SA-anti-Kell and SA-anti-C were also produced.
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Deary et al. (1982) studied this question.
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