What question did this study set out to answer?

To establish and validate a rapid spectrophotometric method for measuring the RNA content and purity of plant viruses by correcting for light scattering.

July 1, 1959Zeitschrift für Naturforschung BOpen Access

Die Bestimmung des Nucleinsäuregehaltes pflanzlicher Viren mit Hilfe einer spektrophotometrischen Methode

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Authors

HPH. L. PaulPhysikalisch-Technische Bundesanstalt

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Implication

Laboratory study demonstrates accurate spectrophotometric quantification of RNA content across nine plant viruses, indicating a reliable method for assessing sample purity.

Key Points

To establish and validate a rapid spectrophotometric method for measuring the RNA content and purity of plant viruses by correcting for light scattering.
Applied the spectrophotometric method of Warburg and Christian across nine distinct plant viruses.
Corrected for virus suspension light scattering using theoretical calculation via Rayleigh's formula or empirical comparison with polystyrene latex suspensions of matching turbidity.
Compared spectrophotometric estimates directly against traditional chemical analyses.
Spectrophotometrically determined RNA values matched closely with amounts ascertained by chemical analysis across all nine tested plant viruses.
Constructed a standardized calibration curve that derives viral RNA content and preparation purity directly from spectrophotometric absorbance measurements.
Formulated a mathematical equation enabling the quantitative spectrophotometric analysis of two-virus mixtures with divergent RNA contents.

Cite This Study

H. L. Paul (1959) studied this question.

synapsesocial.com/papers/6a82933e5b7acbbacb8d5d0c https://doi.org/10.1515/znb-1959-0703

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Implication

Laboratory study demonstrates accurate spectrophotometric quantification of RNA content across nine plant viruses, indicating a reliable method for assessing sample purity.

Die Bestimmung des Nucleinsäuregehaltes pflanzlicher Viren mit Hilfe einer spektrophotometrischen Methode

Discussion

Member takes

Implication

Key Points

Cite This Study

Die Bestimmung des Nucleinsäuregehaltes pflanzlicher Viren mit Hilfe einer spektrophotometrischen Methode

Discussion

Member takes

Implication

Key Points

Cite This Study

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