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June 14, 1977Biochemistry

Separation of quantitation of intracellular forms of poliovirus RNA by agarose gel electrophoresis

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Population

HeLa cells infected with poliovirus

Design

Preclinical

Authors

MHMartinez J. HewlettCalifornia State University SystemSRShmuel RozenblattWeizmann Institute of ScienceVAVictor AmbrosUniversity of Massachusetts Chan Medical School

Discussion

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Implication

Offers no immediate clinical implications; leaves open extension of RNA quantitation to in vivo or therapeutic models.

Structured PICO

P
Population
HeLa cells infected with poliovirus
I
Intervention
Agarose gel electrophoresis (1%) of total cytoplasmic nucleic acids
O
Outcome
Separation and quantitation of single- and double-stranded forms of poliovirus RNAsurrogate

Agarose gel electrophoresis allows for the direct separation and quantitation of single- and double-stranded poliovirus RNA in infected HeLa cells.

Cite This Study

Hewlett et al. (1977) studied this question.

synapsesocial.com/papers/6a77583ef6b553d308ae6eeahttps://doi.org/10.1021/bi00631a027
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs1975 · 86 citations
  2. 2Poliovirus Infection and Poly(A) Sequences of Cytoplasmic Cellular RNA1974 · 16 citations
  3. 3Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties1971 · 151 citations
  4. 4Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis1976 · 120 citations
  5. 5Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis1973 · 1,559 citations