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December 1, 1969European Journal of BiochemistryOpen Access

Replication of Poliovirus RNA Studied by Gel Filtration and Electrophoresis

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Population

Suspension cultures of HeLa cells infected with poliovirus

Design

Preclinical

Follow-up

Up to 5 hours post infection

Authors

BÖB. ÖbergPartners In CareLPLennart PhilipsonLinköping University

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Implication

Advances poliovirus RNA kinetics understanding; extends basic virology but leaves clinical translation open.

Structured PICO

P
Population
Suspension cultures of HeLa cells infected with poliovirus
I
Intervention
Continuous and pulse labeling to study RNA synthesis kinetics, analyzed via gel filtration on sphere-condensed agarose and electrophoresis on polyacrylamide-agarose gels
O
Outcome
Kinetics of synthesis of poliovirus RNA (single stranded RNA, replicative form, and replicative intermediate)surrogate

This basic science study elucidates the kinetics and precursor-product relationships of poliovirus RNA synthesis in HeLa cells.

Cite This Study

Öberg et al. (1969) studied this question.

synapsesocial.com/papers/6a6f57d4ac440176ef2808fehttps://doi.org/10.1111/j.1432-1033.1969.tb00774.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Virus-Specific Double-Stranded RNA in Poliovirus-Infected Cells1964 · 131 citations
  2. 2A Poliovirus-induced Double-stranded RNA: Double-helical Properties and Labelling Kinetics*1965 · 3 citations
  3. 3Characterization of ribonuclease-resistant RNA from poliovirus-infected HeLa cells.1965 · 46 citations
  4. 4Examination of the Qβ-Replicase Reaction by Sucrose Gradient and Gel Electrophoresis1967 · 18 citations
  5. 5Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels1968 · 1,368 citations