June 1, 1989Journal of Biological ChemistryOpen Access

Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis

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Population

Rabbit uterus cDNA clones (SMHC29 and SMHC40) and smooth muscle myosin heavy chain isoforms

Design

Preclinical

Authors

RNR NagaiHeart Failure / Cardiomyopathy MKMakoto Kuro‐oGeneral Cardiology PBPhilip BabijAmgen (United States)

Discussion

Member takes

Implication

Distinguishes SM1/SM2 molecular origins; leaves open differential roles or therapeutic targeting in human vascular disease.

Structured PICO

Population

Rabbit uterus cDNA clones (SMHC29 and SMHC40) and smooth muscle myosin heavy chain isoforms

Intervention

cDNA cloning, nucleotide sequence analysis, and immunoblot analysis using specific antibodies

Outcome

Characterization and identification of smooth muscle myosin heavy chain isoforms (SM1 and SM2) and their corresponding cDNA clonessurrogate

This basic science study demonstrates that two distinct smooth muscle myosin heavy chain isoforms, SM1 and SM2, are encoded by different mRNAs (SMHC40 and SMHC29) that differ at their carboxyl terminus.

Cite This Study

Nagai et al. (1989) studied this question.

synapsesocial.com/papers/6a829e98d38da709b01334d3 https://doi.org/10.1016/s0021-9258(18)81717-3

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Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider