What question did this study set out to answer?

To determine whether additional smooth muscle myosin heavy chain isoform diversity occurs within the globular S1 head region.

Identification of a novel smooth muscle myosin heavy chain cDNA: isoform diversity in the S1 head region

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May alter vascular smooth muscle function in disease; leaves open clinical relevance pending functional studies.

To determine whether additional smooth muscle myosin heavy chain isoform diversity occurs within the globular S1 head region.
Isolated and sequenced two distinct rat smooth muscle myosin heavy chain cDNA clones, designated SMHC-11 (SM1B) and SMHC-5 (SM1A).
Analyzed the tissue-specific distribution and relative proportions of SM1A and SM1B mRNA across various rat smooth muscle tissues using ribonuclease protection assays.
Sequence analysis revealed SM1B is identical to SM1A across the coding region except at the 25/50 kDa junction near the Mg²⁺-ATPase site, where SM1B contains a unique insertion of seven amino acids.
Both isoforms were coexpressed across all smooth muscle tissues tested, with SM1A predominating in most tissues while SM1B was more abundant in the intestine and urinary bladder.

White et al. (1993) studied this question.

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