Improved method for quantitation of B apoprotein in plasma lipoproteins by electroimmunoassay.

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Structured PICO

Population

Plasma lipoproteins (very-low- and low-density lipoprotein spectrum)

Intervention

Pre-treatment of lipoprotein samples with a bacterial lipase prior to electroimmunoassay

Comparator

Standard electroimmunoassay procedures without lipase pre-treatment

Outcome

Quantitation of absolute B apoprotein masssurrogate

Pre-treatment with bacterial lipase improves the reliability of electroimmunoassay for quantifying B apoprotein in plasma lipoproteins.

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May enhance apoB assay reliability in labs; leaves open effects on clinical risk assessment.

Reardon et al. (1981) studied this question.

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