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Side reactions side-stepped: A D-amino acid specific protease was used for the first time in the chemoenzymatic synthesis of a native protein, the peptidyl prolyl cis/trans isomerase parvulin 10 from E. coli (see scheme). Side reactions that complicate synthesis with L-amino acid specific enzymes are avoided in this approach.
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Wehofsky et al. (2008) studied this question.
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