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DEWAN [1938] and Adler et al. [1939] reported that certain enzyme preparations obtained from heart muscle bring about a reductive amination of ac-ketoglutaric acid.The hydrogen required in this reaction may be provided from other dehydrogenase systems via coenzyme I or II, especially from the ,-hydroxy- butyric and i8ocitric dehydrogenases, according to the schemes:(1) ac-ketoglutaric acid+NH3+i8ocitric acid -+glutamic acid + oxalosuccinic acid, or(2) oc-ketoglutaric acid + NH3 + ,-hydroxybutyric acid glutamic acid + acetoacetic acid.We have studied the occurrence of these reactions (so far observed only in artificially combined enzyme systems) in surviving tissues.We find that a reductive amination of oc-ketoglutaric acid does in fact take place in kidney and heart muscle, but this reduction proves independent of the presence of isocitric or fl-hydroxybutyric acids.On the other hand it is found to be accompanied by a formation of succinic acid and C02, and the quantities of these substances formed show that the following reaction takes place:(3) 2oc-ketoglutaric acid + NH3=glutamic acid + succinic acid +C02.
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Krebs et al. (1939) studied this question.
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