How selective antagonists and genetic modification have helped characterise the expression and functions of vascular P2Y receptors

Figures

• 

  Fig. 1. P2Y2 receptors mediate Ca2+ mobilisation in EAhy926 endothelial cells. The mean peak amplitude of responses evoked by UTP (100 nM—30 μM) when two consecutive CRC were constructed per coverslip of cells, first in the absence and then the presence of AR-C118925XX (30 nM) is shown. The data are expressed as a percentage of the response to UTP (10 µM) in the first CRC, n = 5. Vertical lines show SEM. For some points, the error bars are shorter than the height of the symbol. The curves represent the fit of the Hill equation to the data. Note that in control experiments there was no significant change in the EC50 value when two UTP CRC were constructed on the same population of cells in the absence of AR-C118925XX. Reproduced from Muoboghare et al. (2019), with permission from Wiley

• 

  Fig. 2. P2Y2 receptors do not mediate vasoconstriction of the rat intrapulmonary artery. The superimposed traces show typical contractions of the rat isolated intrapulmonary artery evoked by UTP (300 μM) in the absence and presence of AR-C118925XX (1 μM). UTP was added as indicated by the horizontal bar. The mean peak amplitude of contractions evoked by b) UTP (300 μM) and c) ATP (300 μM) in the absence and presence of AR-C118925XX (1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document}μM) is shown. Vertical lines indicate SEM. n = 6 UTP, n = 5 ATP. Reproduced from Dales et al. (2022)

• 

  Fig. 3. P2X1 and P2Y12 receptors mediate vasoconstriction of the rat intrapulmonary artery. a) The superimposed traces show typical contractions of the rat isolated intrapulmonary artery evoked by ATP (300 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document}μM) in the absence (upper trace) and presence (lower trace) of cangrelor (1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document}μM). ATP was applied as indicated by the solid bars. b) The mean peak amplitude of contractions evoked by ATP (300 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document}μM) in the presence of cangrelor (0.1 nM—10…