Key result
A lack of stable RNA secondary structure immediately downstream of the initiator AUG is required for optimal translation initiation and replication of pestivirus RNAs.
Population
Madin-Darby bovine kidney (MDBK) cells and bovine viral diarrhea virus (BVDV) subgenomic replicons
Comparison
Mutations in the NS3 coding sequence or silent… vs Wild-type (wt) MetNS3 RNA
Design
Preclinical
Authors
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May inform pestivirus antiviral design in veterinary models; leaves open extension to human flaviviruses or clinical use.
A lack of stable RNA secondary structure immediately downstream of the initiator AUG is important for optimal translation initiation of pestivirus RNAs.
Myers et al. (2001) studied Bovine Viral Diarrhea Virus (BVDV) infection (in vitro). Mutations altering RNA secondary structure downstream of initiator AUG vs. Wild-type BVDV subgenomic replicon was evaluated on RNA replication and translation efficiency. A lack of stable RNA secondary structure immediately downstream of the initiator AUG is required for optimal translation initiation and replication of pestivirus RNAs.
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