Key result
Mutations in the NS3 and NS4B units were cis dominant and lethal alterations in NS4A and NS5B were noncomplementable, whereas replication of NS5A mutants could be restored in trans.
Population
Transfected host cells with BVDV (bovine viral diarrhea virus) DI9c replicon and its mutant derivatives
Comparison
In-frame mutations introduced into the DI9c open… vs Wild-type or functional DI9c replicon
Design
Preclinical
Authors
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NS protein cis/trans requirements in pestivirus are hypothesis-generating; leaves open extension to flavivirus replication or antiviral targeting.
This study provides initial insights into the organization of the pestivirus replication machinery, demonstrating that while most nonstructural proteins act in cis, NS5A defects can be complemented in trans.
Grassmann et al. (2001) studied Bovine viral diarrhea virus (BVDV) replication. In-frame mutations into the DI9c ORF vs. Wild-type or functional DI9c ORF was evaluated on RNA replication capacity and ability to support translation and cleavage. Mutations in the NS3 and NS4B units were cis dominant and lethal alterations in NS4A and NS5B were noncomplementable, whereas replication of NS5A mutants could be restored in trans.
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