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December 1, 1996Journal of VirologyOpen Access

Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs

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Population

Bovine cells (in vitro model for bovine viral diarrhea virus)

Comparison

Transfection with RNA transcribed in vitro from… vs Parental BVDV and noncytopathogenic counterpart…

Design

Preclinical

Authors

GMGregor MeyersFriedrich-Loeffler-InstitutNTNorbert TautzInstitute for Integrative and Experimental GenomicsPBPaul BecherWilmington College

Discussion

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Implication

Recombination mechanism confirmed for BVDV cytopathogenicity; extends cell-culture observations but leaves open in vivo relevance.

Structured PICO

P
Population
Bovine cells (in vitro model for bovine viral diarrhea virus)
I
Intervention
Transfection with RNA transcribed in vitro from full-length cDNA constructs of BVDV isolate CP7 and its variants
C
Comparator
Parental BVDV and noncytopathogenic counterpart NCP7
O
Outcome
Generation of infectious BVDV and cytopathogenic phenotype

The study demonstrates that recombination-induced alterations in the genomes of cytopathogenic BVDV are responsible for the induction of cell lysis.

Cite This Study

Meyers et al. (1996) studied this question.

synapsesocial.com/papers/6a709898e5469ee92be14cbdhttps://doi.org/10.1128/jvi.70.12.8606-8613.1996
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations
  2. 2Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles1996 · 153 citations
  3. 3Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion1994 · 110 citations
  4. 4Bovine viral diarrhea virus: characterization of a cytopathogenic defective interfering particle with two internal deletions1996 · 44 citations
  5. 5Cytopathogenicity of border disease virus is correlated with integration of cellular sequences into the viral genome1996 · 63 citations