October 1, 2004Antioxidants and Redox Signaling
Role of Oxidative Stress in Ischemia–Reperfusion-Induced Changes in Na + ,K + -ATPase Isoform Expression in Rat Heart
Key result
Treatment of ischemia/reperfusion rat hearts with superoxide dismutase plus catalase preserved the levels of all Na+,K+-ATPase isoforms except alpha1.
Population
Isolated rat hearts
Comparison
Superoxide dismutase plus catalase during… vs Ischemia/reperfusion without SOD+CAT, or…
Design
Preclinical
Follow-up
90 minutes or 20 min perfusion
Authors
Discussion
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Member takes
Implication
Should not alter clinical ischemia/reperfusion management; hypothesis-generating for antioxidant effects on cardiac Na+,K+-ATPase isoforms.
Structured PICO
P
Population
Isolated rat hearts subjected to ischemia/reperfusion to assess Na+,K+-ATPase isoform expression.
I
Intervention
Superoxide dismutase plus catalase (SOD+CAT) during ischemia/reperfusion, or perfusion with 300 microM hydrogen peroxide or 2 mM xanthine plus 0.03 U/ml xanthine oxidase (XXO)
C
Comparator
Ischemia/reperfusion without SOD+CAT, or standard perfusion
O
Outcome
Na+,K+-ATPase alpha1, alpha2, alpha3, beta1, beta2, and beta3 isoform contentssurrogate
Oxidative stress mediates ischemia/reperfusion-induced reductions in most Na+,K+-ATPase isoforms in the rat heart, which can be attenuated by antioxidant treatment.
Cite This Study
Ošťádal et al. (2004) studied Ischemia/reperfusion injury. Superoxide dismutase plus catalase (SOD+CAT) vs. Ischemia/reperfusion without SOD+CAT was evaluated on Na+,K+-ATPase alpha1, alpha2, alpha3, beta1, beta2, and beta3 isoform contents. Treatment of ischemia/reperfusion rat hearts with superoxide dismutase plus catalase preserved the levels of all Na+,K+-ATPase isoforms except alpha1.