Key result
Five amino acids (N470, D271, N272, Y446, and W503) of the AAV9 capsid were identified as required for galactose binding and tissue tropism in the mouse lung.
Population
In vitro cell models and in vivo mouse lung models for testing Adeno-Associated Virus Serotype 9 capsid…
Design
Preclinical
Authors
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May guide AAV9 engineering for targeted gene therapy; leaves open clinical translation from mouse models.
Identification of the specific amino acids required for AAV9 binding to galactose may enable advances in vector engineering for targeted gene therapy.
Bell et al. (2012) studied this question. Site-directed mutagenesis of AAV9 capsid was evaluated on Identification of amino acids required for galactose binding. Five amino acids (N470, D271, N272, Y446, and W503) of the AAV9 capsid were identified as required for galactose binding and tissue tropism in the mouse lung.
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