What question did this study set out to answer?

To determine the cDNA sequence, genomic structure, chromosomal localization, and tissue-specific isoform mechanisms of human dystroglycan.

January 1, 1993Human Molecular Genetics

Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization

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Authors

OIOxana Ibraghimov‐BeskrovnayaVyne Therapeutics (United States)AMAthena MilatovichUniversity of North Carolina at Chapel Hill TÖTayfun ÖzçelıkBilkent University

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Implication

Molecular characterization reveals the genomic organization and chromosomal localization of DAG1 in human tissues, suggesting tissue-specific glycosylation modulates laminin binding.

Key Points

To determine the cDNA sequence, genomic structure, chromosomal localization, and tissue-specific isoform mechanisms of human dystroglycan.
Cloned and characterized human skeletal muscle dystroglycan cDNA.
Mapped the chromosomal location of the human dystroglycan gene (DAG1) and elucidated its genomic exon-intron boundaries.
Analyzed sequence conservation against rabbit dystroglycan and assessed gene expression patterns across fetal and adult human tissues.
Mapped the human dystroglycan gene (DAG1) to chromosome 3 band p21, consisting of two coding exons interrupted by a large intron.
Demonstrated 93% amino acid identity between human and rabbit dystroglycan with conserved predicted glycosylation sites across diverse adult and fetal tissues.
Identified that muscle and non-muscle isoforms share an identical protein core sequence, indicating that tissue-specific isoform differences arise via differential glycosylation.

Cite This Study

Ibraghimov‐Beskrovnaya et al. (1993) studied this question.

synapsesocial.com/papers/6a8317e295f6f1aefa9c8f06 https://doi.org/10.1093/hmg/2.10.1651

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Implication

Molecular characterization reveals the genomic organization and chromosomal localization of DAG1 in human tissues, suggesting tissue-specific glycosylation modulates laminin binding.

Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization

Discussion

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Implication

Key Points

Cite This Study

Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization

Discussion

Member takes

Implication

Key Points

Cite This Study

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