Evidence for 30-40S RNA as Precursor of the 60-70S RNA of Rous Sarcoma Virus

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Population

Rous sarcoma virus propagated in transformed cultures of chick fibroblasts

Comparison

Harvesting virus at 3-minute intervals and… vs Virus harvested at hourly intervals or…

Design

Preclinical

Authors

Discussion

Demonstrates 30-40S RNA precursor role in RSV; leaves open in vivo replication and oncogenesis relevance.

Population

Rous sarcoma virus (Prague strain-C and Schmidt-Ruppin strain-A) propagated in transformed cultures of chick fibroblasts

Intervention

Harvesting virus at 3-minute intervals and subsequent incubation at 40 degrees Celsius

Comparator

Virus harvested at hourly intervals or unincubated virus

Outcome

RNA sedimentation coefficient and conversion of 30-40S RNA to 60-70S RNAsurrogate

This basic science study demonstrates that the 30-40S RNA of Rous sarcoma virus serves as a physical precursor to the 60-70S RNA complex.

Canaani et al. (1973) studied this question.

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