What question did this study set out to answer?

To examine the regulation of heme biosynthesis and determine the mechanisms underlying the induction of δ-aminolevulinic acid synthetase in cultured liver cells.

May 1, 1972Journal of Biological ChemistryOpen Access

The Induction of δ-Aminolevulinic Acid Synthetase in Cultured Liver Cells

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Authors

LSLars StrandUniversity of Southern California JMJoan A. ManningUniversity of California, San Francisco HMHarvey S. MarverUniversity of California, San Francisco

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Implication

In vitro study demonstrates distinct pathways of δ-aminolevulinic acid synthetase induction in avian hepatocytes, highlighting heme-mediated feedback repression relevant to acute porphyria.

Key Points

To examine the regulation of heme biosynthesis and determine the mechanisms underlying the induction of δ-aminolevulinic acid synthetase in cultured liver cells.
Assayed δ-aminolevulinic acid synthetase activity in cultured avian hepatocytes exposed to 2-allyl-2-isopropylacetamide and lead acetate.
Evaluated enzyme induction in the presence of transcriptional protein synthesis inhibitors, exogenous heme, and the precursor δ-aminolevulinic acid.
Both 2-allyl-2-isopropylacetamide and lead acetate induced δ-aminolevulinic acid synthetase synergistically, but transcriptional inhibitors blocked induction by 2-allyl-2-isopropylacetamide and not lead acetate.
Treatment with exogenous heme and δ-aminolevulinic acid prevented enzyme induction by both compounds, indicating that heme functions as a natural repressor acting at a translational site.

Cite This Study

Strand et al. (1972) studied this question.

synapsesocial.com/papers/6a83337928b8a62be9990baa https://doi.org/10.1016/s0021-9258(19)45284-8

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Implication

In vitro study demonstrates distinct pathways of δ-aminolevulinic acid synthetase induction in avian hepatocytes, highlighting heme-mediated feedback repression relevant to acute porphyria.

The Induction of δ-Aminolevulinic Acid Synthetase in Cultured Liver Cells

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Member takes

Implication

Key Points

Cite This Study

The Induction of δ-Aminolevulinic Acid Synthetase in Cultured Liver Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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