Filtration or sodium deoxycholate treatment can resolve viral aggregation that interferes with correct identification and neutralization of rhinovirus.
Hypothesis-generating for rhinovirus assay standardization; leaves open effects on clinical diagnostics or vaccine assessment.
A seed lot of strain SF 1684 of rhinovirus type 2 prepared in human embryonic lung cells (WI-38) contained aggregates which interfered with its neutralization by homotypic or homologous antisera. The same virus showed no evidence of aggregation at five other passage levels studied. Virus in the seed lot was not identified correctly, and the titer of homologous antiserum was mistakenly considered to be low as a result of neutralization tests conducted with the aggregated virus. Filtration and a more easily effected treatment with sodium deoxycholate (1%) disaggregated the virus and restored its susceptibility to neutralization by homologous and homotypic antiserum.
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Gwaltney et al. (1970) studied this question.
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