Glyburide, a KATP Channel Antagonist, Attenuates the Cardioprotective Effects of Isoflurane in Stunned Myocardium

Dogs (n=14) chronically instrumented for measurement of left ventricular and aortic blood pressure, cardiac output, coronary blood flow velocity, and subendocardial segment length, subjected to 15-min left anterior descending coronary artery occlusion (LAD) and reperfusion.

Glyburide (0.3 mg/kg, intravenously) pretreatment before 2% isoflurane anesthesia and 15-min LAD occlusion.

Drug vehicle pretreatment before 2% isoflurane anesthesia and 15-min LAD occlusion.

Recovery of myocardial contractile function (segment shortening, preload recruitable work area [PRWA], and time constant of isovolumic relaxation [tau]) at 5 hours postreperfusion.surrogate