October 31, 2000Proceedings of the National Academy of SciencesOpen Access

Chimeras of Dictyostelium myosin II head and neck domains with Acanthamoeba or chicken smooth muscle myosin II tail domain have greatly increased and unregulated actin-dependent MgATPase activity

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Population

Chimeric myosins expressed in Dictyostelium myosin II heavy chain-null cells

Comparison

Phosphorylation of regulatory light chain and… vs Wild-type Dictyostelium myosin II

Design

Preclinical

Authors

XLXiong LiuGuiyang Medical University SSShi ShuHohai University RYRoxanne A. YamashitaNational Institutes of Health

Discussion

Member takes

Implication

Chimeric myosin findings in Dictyostelium leave open whether head-tail domain coupling governs regulation in mammalian cardiac myocytes; hypothesis-generating only.

Structured PICO

Population

Chimeric myosins (Dictyostelium myosin II head/neck fused with Acanthamoeba or chicken smooth muscle myosin II tail) expressed in Dictyostelium myosin II heavy chain-null cells

Intervention

Phosphorylation of regulatory light chain (RLC) and heavy chain

Comparator

Wild-type Dictyostelium myosin II

Outcome

Actin-dependent MgATPase activity (Vmax and KATPase)surrogate

Chimeric class II myosins exhibit greatly increased and unregulated actin-dependent MgATPase activity, indicating that normal regulation requires specific, tightly coupled interactions between head and tail domains.

Limitations

Potential difficulty in interpreting KATPase and Vmax data for multiheaded filamentous myosins

Cite This Study

Liu et al. (2000) studied this question.

synapsesocial.com/papers/6a83584ab2e3e1ac79a6e8cd https://doi.org/10.1073/pnas.230441497

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Implication

Chimeric myosin findings in Dictyostelium leave open whether head-tail domain coupling governs regulation in mammalian cardiac myocytes; hypothesis-generating only.

Chimeras of Dictyostelium myosin II head and neck domains with Acanthamoeba or chicken smooth muscle myosin II tail domain have greatly increased and unregulated actin-dependent MgATPase activity

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Chimeras of Dictyostelium myosin II head and neck domains with Acanthamoeba or chicken smooth muscle myosin II tail domain have greatly increased and unregulated actin-dependent MgATPase activity

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider