February 1, 1985Journal of Biological ChemistryOpen Access

Filament formation and actin-activated ATPase activity are abolished by proteolytic removal of a small peptide from the tip of the tail of the heavy chain of Acanthamoeba myosin II.

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Population

Myosin II from Acanthamoeba castellanii

Comparison

Proteolytic cleavage by chymotrypsin to remove a… vs Undigested native myosin II

Design

Preclinical

Authors

JKJacek KuźnickiInternational Institute of Molecular and Cell Biology GCGraham P. CôtéRELX Group (Netherlands)BBBlair BowersNational Institutes of Health

Discussion

Member takes

Implication

Myosin tail segment required for filament assembly and Mg-ATPase; hypothesis-generating for cardiac regulation, needs mammalian validation before any clinical consideration.

Structured PICO

Population

Myosin II from Acanthamoeba castellanii

Intervention

Proteolytic cleavage by chymotrypsin to remove a small peptide containing three phosphorylation sites from the ends of the myosin II heavy chains

Comparator

Undigested native myosin II

Outcome

Filament formation and actin-activated Mg2+-ATPase activitysurrogate

The short segment near the end of the myosin II tail containing three phosphorylatable serines is necessary for bipolar filament formation and subsequent actin-activated Mg2+-ATPase activity.

Cite This Study

Kuźnicki et al. (1985) studied this question.

synapsesocial.com/papers/6a8a9fc6755d28965dfd75ec https://doi.org/10.1016/s0021-9258(18)89686-7

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Implication

Myosin tail segment required for filament assembly and Mg-ATPase; hypothesis-generating for cardiac regulation, needs mammalian validation before any clinical consideration.

Filament formation and actin-activated ATPase activity are abolished by proteolytic removal of a small peptide from the tip of the tail of the heavy chain of Acanthamoeba myosin II.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Filament formation and actin-activated ATPase activity are abolished by proteolytic removal of a small peptide from the tip of the tail of the heavy chain of Acanthamoeba myosin II.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider