Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 1, 1993Biochemistry

Transient detection of spin-labeled myosin subfragment 1 conformational states during ATP hydrolysis

View Full Paper
Ask AI
Bookmark
Share

Population

Spin-labeled myosin heads (IASL-S1)

Design

Preclinical

Authors

EOE. Michael OstapPenn Center for AIDS ResearchHWHoward D. WhiteHeart Failure & TransplantDTDavid D. ThomasHeart Failure / Cardiomyopathy

Discussion

Loading...

Member takes

Implication

Supports mechanistic studies of myosin dynamics; leaves open translation to cardiac muscle or human therapeutics.

Structured PICO

P
Population
Spin-labeled myosin heads (IASL-S1)
I
Intervention
Photolysis of caged ATP (producing 500 microM ATP)
O
Outcome
Nucleotide-induced changes in the conformational state and internal rotational dynamics of spin-labeled myosin headssurrogate

This study establishes the feasibility of directly detecting and resolving myosin's conformational transients during muscle contraction using time-resolved EPR spectroscopy.

Cite This Study

Ostap et al. (1993) studied this question.

synapsesocial.com/papers/6a83585437400aad4b18f577https://doi.org/10.1021/bi00077a026
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Effects of Various Nucleotides on the Structure of Actin-Attached Myosin Subfragment-1 Studied by Quick-Freeze Deep-Etch Electron Microscopy11989 · 55 citations
  2. 2Effect of nucleotides and pyrophosphate on spin labels bound to S1 thiol groups of myosin1970 · 93 citations
  3. 3Suppression of muscle contraction by vanadate. Mechanical and ligand binding studies on glycerol-extracted rabbit fibers.1985 · 79 citations
  4. 4Small-Angle Synchrotron X-Ray Scattering Reveals Distinct Shape Changes of the Myosin Head During Hydrolysis of ATP1992 · 121 citations
  5. 5Ligand-induced myosin subfragment 1 global conformational change1990 · 38 citations