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Isolate, map, and molecularly characterize the gene encoding myosin light chain two (MLC2) in Drosophila melanogaster.

Isolation and characterization of the gene for myosin light chain two of Drosophila melanogaster.

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Establishes Drosophila MLC2 as a single-locus model for isoform diversity; leaves open translation to human cardiac myosin regulation.

Isolate, map, and molecularly characterize the gene encoding myosin light chain two (MLC2) in Drosophila melanogaster.
Screened a recombinant lambda-phage library of randomly sheared Drosophila genomic DNA to isolate the MLC2 gene.
Identified the gene product using in vitro translation and two-dimensional gel electrophoresis compared against native muscle proteins.
Evaluated developmental RNA expression profiles in embryos and adult thoracic muscle, and determined the cDNA nucleotide sequence.
The MLC2 gene was localized to chromosomal region 99E1-3 on chromosome 3, separate from the MLC1 locus at 98B, and accounted for all larval and thoracic MLC2 isoforms.
MLC2 transcripts were specifically detected following the onset of muscle differentiation in embryos and were highly abundant in adult thoracic muscle.
The translated protein sequence demonstrated extensive homology to vertebrate MLC2 and featured an acetylated amino-terminus.

Toffenetti et al. (1987) studied this question.

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