Studies on the Histochemical Specificity of Esterase

Pig pancreas extract is examined by means of paper chromatographic technic using 1.5% NaCl and 10% acetone buffer solution pH 5.0 as a developer. Esterase and lipase are separated and they showed color reactions in different places respectively. At least two spots are found in esterase reaction.2. Eleven kinds of effectors are applied to the separated esterase portion on paper chromatogram and resulting color reactions are examined. At least in two spots, different color reactions are noted, depending on the different substances applied Atoxyl, Eserine and Thyroxine showed strong color reaction in the spot of relative larger Rf value. Fluoride, Urethane, Qunine, Cocaine and Nitromin showed weak color reaction (inhibition) in the spot of smaller Rf value. Strychinine and 1-Leucylglycylglycine showed inhibition in both spots in same degree. Taurocholate showed activation in both spots.