Immunohistochemical Studies on Malignant Neoplasms by Means of Fluorescent Antibody Methods

Recently. it became clear that certain mouse tumors were induced by viruses and the viruses were morphologically demonstrated by electron microscopy. The fluorescent antibody methods2) have been utilized to persue the localization of viral antigens. Many studies in this field have been performed by investigators: namely observations on viral antigen of Rous sarcoma by Mellors et al10), Malmgren et al 8), Vogt et al 14) and Noyes 13), investigations on Shope papilloma by Noyes12) and on Polyoma by Malmgren 9). In Japan. Chicken erythroblastosis virus was investigated by Iwakata6) and mouse leukemia virus by Ichikawa et al5) as studied with fluorescent antibody method. The purpose of this communication is to present a study on localization of viral antigens in C3H strain mouse mammary cancer and C 58 and AKR strain mouse leukemias, and an observation on the site of antibody production in hyperimmune rabbit.