Binding Sites for Atrial Natriuretic Peptide in High Concentrations in Human Ovary

Four human ovaries (one with a 7 mm developing follicle, two with preovulatory follicles of 12 mm and 18 mm, and one at menopause) obtained from patients with uterine myoma or carcinoma in situ during abdominal total hysterectomy with tubo-oophorectomy.

Radioligand assay and affinity labeling using 125I-rANP to examine binding capacities and dissociation constants.

Binding capacity (Bmax) and dissociation constant (Kd) of ANP binding sites in human ovaries at different stages of follicular development.surrogate