December 15, 1996Journal of Clinical InvestigationOpen Access

Growth inhibitory properties of endothelin-1 in activated human hepatic stellate cells: a cyclic adenosine monophosphate-mediated pathway. Inhibition of both extracellular signal-regulated kinase and c-Jun kinase and upregulation of endothelin B receptors.

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Population

Activated human hepatic stellate cells obtained by outgrowth from explants of normal liver obtained after…

Comparison

Endothelin-1, ETB receptor agonist… vs Untreated control cells or vehicle

Design

Preclinical

Authors

AMAriane MallatUniversity of East Anglia APAnne-Marie PréauxInserm CGC. Serradeil‐Le GalUniversity of Pecs

Discussion

Member takes

Implication

Hypothesis-generating for cAMP-elevating agents in hepatic fibrosis; leaves open translation to human chronic liver disease.

Structured PICO

Population

Activated human hepatic stellate cells (HSC) obtained by outgrowth from explants of normal liver obtained after surgery of benign or malignant liver tumors

Intervention

Endothelin-1 (ET-1), ETB receptor agonist sarafotoxin-S6C, forskolin, and prostaglandins (PGE2, PGI2)

Comparator

Untreated control cells or vehicle

Outcome

Cell proliferation (DNA synthesis measured by [3H]thymidine incorporation), cAMP levels, ERK and JNK activity, and ETB receptor expressionsurrogate

ET-1 inhibits the proliferation of activated human hepatic stellate cells via ETB receptors through a prostaglandin/cAMP pathway, suggesting that agents increasing cAMP might help limit HSC proliferation in chronic liver diseases.

Cite This Study

Mallat et al. (1996) studied this question.

synapsesocial.com/papers/6a836f39df262e78b0720e8f https://doi.org/10.1172/jci119103

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Growth inhibitory properties of endothelin-1 in activated human hepatic stellate cells: a cyclic adenosine monophosphate-mediated pathway. Inhibition of both extracellular signal-regulated kinase and c-Jun kinase and upregulation of endothelin B receptors.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Growth inhibitory properties of endothelin-1 in activated human hepatic stellate cells: a cyclic adenosine monophosphate-mediated pathway. Inhibition of both extracellular signal-regulated kinase and c-Jun kinase and upregulation of endothelin B receptors.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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