April 2, 2010PLoS ONEOpen Access

ERK1/2 Signaling Dominates Over RhoA Signaling in Regulating Early Changes in RNA Expression Induced by Endothelin-1 in Neonatal Rat Cardiomyocytes

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Population

Neonatal (1-2 days old) Sprague-Dawley rat ventricular cardiomyocytes

Comparison

Endothelin-1 for 1 hour with or without PD184352… vs Unstimulated cardiomyocytes (Controls)

Design

Preclinical

Follow-up

1 hour

Authors

AMA. MarshallUniversity of Bristol OBOliver BarrettMRC Laboratory of Molecular Biology TCTimothy E. CullingfordImperial College London

Discussion

Member takes

Implication

Does not yet inform clinical hypertrophy management; leaves open ERK1/2 as therapeutic node pending human and in vivo validation.

Structured PICO

Population

Neonatal (1-2 days old) Sprague-Dawley rat ventricular cardiomyocytes

Intervention

Endothelin-1 (100 nM) for 1 hour with or without PD184352 (2 mM, ERK1/2 inhibitor) or C3 transferase (C3T, 1 or 5 mg/ml, RhoA inhibitor)

Comparator

Unstimulated cardiomyocytes (Controls)

Outcome

Early changes in RNA expression (transcriptomic response) measured by microarrays and qPCRsurrogate

In neonatal rat cardiomyocytes, ERK1/2 signaling dominates over RhoA in regulating the early transcriptomic response to endothelin-1, identifying it as a key node in early cardiomyocyte hypertrophy.

Cite This Study

Marshall et al. (2010) studied this question.

synapsesocial.com/papers/6a83835efe0f11dd66d103c8 https://doi.org/10.1371/journal.pone.0010027

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ERK1/2 Signaling Dominates Over RhoA Signaling in Regulating Early Changes in RNA Expression Induced by Endothelin-1 in Neonatal Rat Cardiomyocytes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

ERK1/2 Signaling Dominates Over RhoA Signaling in Regulating Early Changes in RNA Expression Induced by Endothelin-1 in Neonatal Rat Cardiomyocytes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider