What question did this study set out to answer?

To assess platelet membrane fluidity, thrombin responsiveness, and membrane lipid composition in patients with poorly controlled diabetes compared to healthy individuals.

February 1, 1990Diabetes

Reduced Membrane Fluidity in Platelets From Diabetic Patients

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Population

24 subjects (12 poorly controlled diabetic subjects and 12 age- and sex-matched control subjects)

Design

Case-control

Authors

PWP.D. WinocourCancer Research UK MBMaria BryszewskaUniversity of Łódź CWCezary WatałaMedical University of Lodz

Discussion

Member takes

Implication

May contribute to platelet hypersensitivity in diabetes; hypothesis-generating and should not yet change practice.

Key Points

To assess platelet membrane fluidity, thrombin responsiveness, and membrane lipid composition in patients with poorly controlled diabetes compared to healthy individuals.
Conducted a case-control comparison of 12 poorly controlled diabetic subjects and 12 age- and sex-matched control subjects (N=24).
Measured platelet activation via [14C]serotonin release after stimulation with low-concentration thrombin.
Assessed membrane fluidity using steady-state fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene (DPH) alongside plasma and platelet lipid analysis.
Platelets from diabetic subjects exhibited significantly higher mean steady-state fluorescence polarization than controls, demonstrating decreased membrane fluidity.
Mean [14C]serotonin release in response to thrombin was not significantly different overall between groups, though 8 of 12 diabetic subjects showed greater release than their matched controls.
The plasma ratio of (VLDL + LDL) to (HDL2 + HDL3) was significantly elevated in diabetic subjects, while the total platelet cholesterol-phospholipid molar ratio showed no significant difference.

Structured PICO

Population

24 subjects (12 poorly controlled diabetic subjects and 12 age- and sex-matched control subjects)

Comparator

Age- and sex-matched control subjects

Outcome

Platelet response to thrombin, platelet membrane fluidity, and platelet cholesterol-phospholipid molar ratiosurrogate

Platelets from poorly controlled diabetic patients exhibit decreased membrane fluidity, which may contribute to their hypersensitivity to agonists like thrombin.

Cite This Study

Winocour et al. (1990) studied this question.

synapsesocial.com/papers/6a838892cfeb7a169036add7 https://doi.org/10.2337/diab.39.2.241

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Reduced Membrane Fluidity in Platelets From Diabetic Patients

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Reduced Membrane Fluidity in Platelets From Diabetic Patients

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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